Journal: Physiological Genomics

Article Title: Circular RNAs in rat models of cardiovascular and renal diseases

doi: 10.1152/physiolgenomics.00064.2017

Figure Lengend Snippet: Box plots show the distribution of expression levels for all samples in the circular (circ)RNA microarray data set. R-1, R-2, and R-3 are 3 individual Dahl salt-resistant rats (R). S-1, S-2, and S-3 are 3 individual Dahl salt-sensitive rats (S). WKY-1, WKY-2, and WKY-3 are 3 individual Wistar Kyoto rats (WKY). SHR-1, SHR-2, and SHR-3 are 3 individual spontaneously hypertensive rats (SHR).

Article Snippet: Arraystar Rat Circular RNA Microarray ( www.arraystar.com ) was used for microarray experiments.

Techniques: Expressing, Microarray

Journal: Physiological Genomics

Article Title: Circular RNAs in rat models of cardiovascular and renal diseases

doi: 10.1152/physiolgenomics.00064.2017

Figure Lengend Snippet: Differentially expressed circRNAs between normotensive and hypertensive rats. The figure represents the number of circRNAs differentially expressed according to the microarray analysis. Based on the location and direction on the genome, circRNAs are subcategorized into exonic, intronic, antisense, sense overlapping, and intergenic. S vs. R up indicates circRNAs were upregulated in Dahl salt-sensitive rats (S) compared with Dahl salt-resistant rats (R). S vs. R down indicates circRNAs were downregulated in S compared with R. SHR vs. WKY up indicates circRNAs were upregulated in spontaneously hypertensive rats (SHR) compared with Wistar Kyoto rats (WKY). SHR vs. WKY down indicates circRNAs were downregulated in SHR compared with WKY.

Article Snippet: Arraystar Rat Circular RNA Microarray ( www.arraystar.com ) was used for microarray experiments.

Techniques: Microarray

Journal: Physiological Genomics

Article Title: Circular RNAs in rat models of cardiovascular and renal diseases

doi: 10.1152/physiolgenomics.00064.2017

Figure Lengend Snippet: Validation of differentially expressed circRNAs in the microarray study by quantitative RT-PCR. R, Dahl salt-resistant rat; S, Dahl salt-sensitive rat; WKY, Wistar Kyoto rat; SHR, spontaneously hypertensive rat. *P < 0.05, **P < 0.01, ***P < 0.001.

Article Snippet: Arraystar Rat Circular RNA Microarray ( www.arraystar.com ) was used for microarray experiments.

Techniques: Biomarker Discovery, Microarray, Quantitative RT-PCR

Journal: Journal of Cellular and Molecular Medicine

Article Title: Identification of circRNA and mRNA expression profiles and functional networks of vascular tissue in lipopolysaccharide‐induced sepsis

doi: 10.1111/jcmm.15424

Figure Lengend Snippet: Validation of circRNA and mRNA expression. A, The relative expression levels of selected circRNAs, as detected by RT‐qPCR. B, The relative expression levels of selected mRNAs, as detected by RT‐qPCR. β‐actin was used as a housekeeping gene for normalizing changes in specific gene expression. * P < .05 and ** P < .01 vs control group, n = 5 per group. RT‐PCR, real‐time quantitative polymerase chain reaction

Article Snippet: The labelled cRNAs were hybridized onto the Arraystar Rat circRNA Array (8 × 15K; Arraystar).

Techniques: Expressing, Quantitative RT-PCR, Reverse Transcription Polymerase Chain Reaction, Real-time Polymerase Chain Reaction

Journal: Physiological Genomics

Article Title: Circular RNAs in rat models of cardiovascular and renal diseases

doi: 10.1152/physiolgenomics.00064.2017

Figure Lengend Snippet: Heat maps of differentially expressed circRNAs between normotensive and hypertensive rats. A: Dahl salt-sensitive rats (S) vs. Dahl salt-resistant rats (R). B: spontaneously hypertensive rats (SHR) vs. Wistar Kyoto rats (WKY). The differentially expressed circRNAs were selected with a P value cut-off 0.05 and a fold-change cut-off 1.5. CircRNA expression level is shown as a function of color, with lower expression in green and higher expression in red. R-1, R-2, and R-3 are 3 individual R rats. S-1, S-2, and S-3 are 3 individual S rats. WKY-1, WKY-2, and WKY-3 are 3 individual WKY rats. SHR-1, SHR-2, and SHR-3 are 3 individual SHR rats.

Article Snippet: The labeled cRNAs were hybridized onto the Arraystar Rat CircRNA Array (8x15K) and incubated for 17 h at 65°C in an Agilent hybridization oven.

Techniques: Expressing

Journal: Physiological Genomics

Article Title: Circular RNAs in rat models of cardiovascular and renal diseases

doi: 10.1152/physiolgenomics.00064.2017

Figure Lengend Snippet: Targeted circRNA-micro (mi)RNA-gene network and functional pathway analysis for rno_circRNA_006016. A: the circRNA-miRNA-gene network based on bioinformatics analysis. Only top 30 miRNA target genes for each miRNA are shown in the network. B: the top 10 significant Gene Ontology for Biological Processes (GO:BP) were predicted by using all the 5 miRNA target genes. C: the top 10 significant Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways were predicted by using all 5 miRNA target genes. “Enrichment Score” = “−log10(P value)” and P value < 0.05 was considered as statistically significant.

Article Snippet: The labeled cRNAs were hybridized onto the Arraystar Rat CircRNA Array (8x15K) and incubated for 17 h at 65°C in an Agilent hybridization oven.

Techniques: Functional Assay